Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates. Results: Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9, or blaCTX-M-8 were found to be genetically unrelated. Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.Fil: Vignoli, Rafael. Universidad de la República; UruguayFil: García Fulgueiras, Virginia. Universidad de la República; UruguayFil: Cordeiro, Nicolás F.. Universidad de la República; UruguayFil: Bado, Inés. Universidad de la República; UruguayFil: Seija, Verónica. Universidad de la República; Uruguay. Hospital Pasteur de Montevideo; UruguayFil: Aguerrebere, Paula. Universidad de la República; UruguayFil: Laguna, Gabriel. Universidad de la República; UruguayFil: Araújo, Lucía. Universidad de la República; UruguayFil: Bazet, Cristina. Universidad de la República; UruguayFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Chabalgoity Rodríguez, José Alejandro. Universidad de la República; Urugua

Optimization of hospital communication in social networks. Analysis and engagement strategies

La comunicación hospitalaria tiende cada vez más a maximizar los beneficios de la gestión de redes sociales y su engagement. Para lograr este objetivo, es clave el estudio analítico. Con el objetivo de establecer recomendaciones centradas en optimizar la estrategia de contenidos en las redes sociales de hospitales e identificar elementos de mejora, se presentan resultados obtenidos con la ayuda de diversas herramientas analíticas aplicadas a una muestra de centros hospitalarios de Alicante (España). Con base en el registro actual de la base de datos ObservaTICs, son descritos los perfiles de Facebook y Twitter, utilizando ratios para la medición del engagement y a través de la revisión del tráfico de sus páginas web. En conclusión, se observa que los centros, si bien pueden mostrar diferencias en cuanto a su actividad y calidad de contenido, requieren una planificación y control de las redes sociales para comunicarse con sus públicos, sus stakeholders y facilitar información útil sobre asuntos de salud que repercute en la reputación de la propia institución y especialmente en la salud de los mismos pacientes. En el futuro es probable que se incremente este tipo de comunicación mediante contenidos enfocados a reforzar los vínculos paciente-médico, así como paciente-hospital.Hospital communication tends more and more to maximize the benefits of social media management and engagement. To achieve this goal, analytical study is key. With the aim of establishing recommendations focused on optimizing the content strategy in hospital social networks and identifying elements for improvement, results obtained with the help of various analytical tools applied to a sample of hospital centers in Alicante (Spain) are presented. Based on the current registry of the ObservaTICs database, the Facebook and Twitter profiles are described, using ratios to measure engagement and through the traffic review of their web pages. In conclusion, it is observed that the centers, although they may show differences in terms of their activity and quality of content, require planning and control of social networks to communicate with their audiences, their stakeholders and provide useful information on health matters that It has repercussions on the reputation of the institution itself and especially on the health of the patients themselves. In the future, this type of communication is likely to increase through content focused on reinforcing patient-doctor links, as well as patient-hospital.Esta investigación forma parte del Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020. Proyectos de I+D+i «Retos investigación» (RTI2018-095714-B-C21). Ministerio de Educación, Gobierno de España